Figure 2

dbcAMP reverses ET-1 (1 nmol/L) -induced vasoconstriction in a dose-dependent manner. A) A representative recording of [Ca²⁺]_i-diameter measurements. The [Ca²⁺]_i was normalized to the value prior to application of ET-1 (value at timepoint "x" was set to 1.0). Maximal vasodilation was determined by exposure of the SMA to Ca-free conditions ("0Ca"). An increase of extracellular Ca²⁺ to 10 mmol/L ("Ca") induced a [Ca²⁺]_i increase and parallel vasoconstriction. B) Dose-response curves of dbcAMP-induced vasodilation and [Ca²⁺]_i decrease of ET-1 (1 nmol/L) preconstricted SMA. C) dbcAMP prevented ET-1-induced Ca²⁺-sensitization.